First Appearance: X-Men #1 (1963)
Magneto is one of the most complex villains in comics. He's a Holocaust survivor and the most powerful mutant in the Marvel Universe. He's sympathetic figure who wants to do good for all mutants but does have evil intentions to wipe out the human race.
From: The Adventures of Tintin
First Appearance: Tintin in the Land of the Soviets (1929)
Tintin is a journalist who travels around the world investigating any and all news stories. What makes Tintin so influential is he's overall good in nature and seeks adventure, which fans of the series can identify with him.
23. James Bond
From: James Bond
First Appearance: Casino Royale (1953)
James Bond is the ultimate spy in fiction and he's the biggest influence in the spy genre. The original Ian Fleming novel series are still in print and the film series is more popular now than any time before.
22. The Joker
First Appearance: Batman #1 (1940)
Consider to be one of the greatest villains in fiction, The Joker is the greatest rival of Batman. Shocking Batman fans since 1940 and with no sign of stopping, The Joker is one of those villains people love to hate.
21. James T. Kirk
From: Star Trek
First Appearance: The Man Trap (1966)
Noble captain of the starship USS Enterprise, James Tiberius Kirk is one of Sci-Fi's most beloved and influential character in the genre. Kirk is best known for his leadership and loyalty to his crew.